Biotin Anti-Human MICA/MICB (6D4)

MICA/MICB Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2a, kappa






Biotin Fluorescent Dye



Cat no : Biotin-65161



Tested Applications

Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

Biotin-65161 targets MICA/MICB in IHC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Transfected C1R cells expressing MICA
Full Name MHC class I polypeptide-related sequence A
Calculated molecular weight 383 aa, 43 kDa
GenBank accession numberBC016929
Gene symbol MICA
Gene ID (NCBI) 4276
Conjugate Biotin Fluorescent Dye
Form Liquid
Purification Method The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.


Product Specific Protocols
IHC protocol for Biotin MICA/MICB antibody Biotin-65161Download protocol
Standard Protocols
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