Biotin Anti-Human MICA/MICB (6D4)

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  IHC staining of human tonsillitis using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using Biotin-65161

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using Biotin-65161 (MICA/MICB antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using Biotin-65161

  1x10^6 HeLa cells were surface stained with 0.5 ug Biotin Anti-Human MICA/MICB (6D4) (Biotin-65161, Clone:6D4) or isotype control, and SA-PE. Cells were not fixed.

MICA/MICB Monoclonal Antibody for IHC, FC

Cat No. Biotin-65161

Clone No.6D4

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

human

Applications

IHC, FC

Synonyms

MICA, MIC-A, MIC A, 6D4

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-65161 targets MICA/MICB in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Transfected C1R cells expressing MICA
• Full Name: MHC class I polypeptide-related sequence A
• Calculated Molecular Weight: 383 aa, 43 kDa
• GenBank Accession Number: BC016929
• Gene Symbol: MICA
• Gene ID (NCBI): 100507436
• RRID: AB_2883067
• Conjugate: Biotin
• Form: Liquid
• Purification Method: The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
• UNIPROT ID: Q29983
• Storage Buffer: PBS with 0.09% sodium azide , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Human MHC class I chain-related genes located within the HLA class I region of chromosome 6 encode MHC class I chain-related A and B (MICA and MICB) (PMID: 11429322). MICA and MICB are stress-inducible surface molecules that are not associated with β2-microglobulin and do not present peptides (PMID: 9497295). They are expressed in intestinal epithelium and many epithelial tumors (PMID: 10359807). MICA and MICB are ligands for NKG2D which is an activating receptor that is expressed on most natural killer (NK) cells, CD8 αβ T cells, and γδ T cells (PMID: 10426993; 11491531). The monoclonal antibody 6D4 recognizes MICA and MICB.