CoraLite® Plus 488-conjugated MGP Monoclonal antibody

MGP Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A1C3

Cat no : CL488-60055

Synonyms

GIG36, matrix Gla protein, MGLAP, MGP, NTI

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Validation Data Gallery

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  IF Staining of MDA-MB-231 using CL488-60055

  Immunofluorescent analysis of (4% PFA) fixed MDA-MB-231 cells using CoraLite® Plus 488 MGP antibody (CL488-60055, Clone: 1A1C3 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: MDA-MB-231 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60055 targets MGP in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MGP fusion protein Ag1091
• Full Name: matrix Gla protein
• Calculated Molecular Weight: 103 aa, 13 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC005272
• Gene Symbol: MGP
• Gene ID (NCBI): 4256
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Matrix Gla protein (MGP) is is a vitamin K-dependent, extracellular matrix protein. MGP plays a pivotal role in preventing soft tissue calcification and local mineralization of the vascular wall. Vitamin K deficiency leads to inactive uncarboxylated MGP (ucMGP), which accumulates at sites of arterial calcification. However MGP is synthesized in many tissues and is especially enriched in embryonic tissues and in cancer cells. Defects in MGP are the cause of Keutel syndrome (KS), which is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.