CoraLite® Plus 488-conjugated MFF Monoclonal antibody

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  IF Staining of mouse brain using CL488-66527

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 MFF antibody (CL488-66527, Clone: 1G4B12 ) at dilution of 1:200.

MFF Monoclonal Antibody for IF-P

Cat No. CL488-66527

Clone No.1G4B12

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

IF-P

Synonyms

AD033, C2orf33, DKFZp666J168, GL004, MFF, mitochondrial fission factor

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66527 targets MFF in IF-P applications and shows reactivity with Human, pig, rat, mouse samples.

• Tested Reactivity: Human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MFF fusion protein Ag9990
• Full Name: mitochondrial fission factor
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: BC000797
• Gene Symbol: MFF
• Gene ID (NCBI): 56947
• RRID: AB_2919338
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9GZY8
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.