CoraLite® Plus 488-conjugated MFF Polyclonal antibody

• Featured Product
• KD/KO Validated

MFF Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17090

Synonyms

AD033, C2orf33, DKFZp666J168, GL004, MFF, mitochondrial fission factor

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-17090

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 MFF antibody (CL488-17090) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-17090 targets MFF in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFF fusion protein Ag9990
• Full Name: mitochondrial fission factor
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 26-29 kDa, 35-38 kDa
• GenBank Accession Number: BC000797
• Gene Symbol: MFF
• Gene ID (NCBI): 56947
• RRID: AB_2919144
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MFF (mitochondrial fission factor) is a mitochondrial outer membrane protein that is involved in mitochondrial localization of Drp1 and mitochondrial fission. Multiple isoforms of MFF exist due to the alternative splicing. This antibody recognizes the endogenous MFF protein around 26-29 kDa and 35-38 kDa.