CoraLite®594-conjugated METTL3 Monoclonal antibody

• Featured Product
• KD/KO Validated

METTL3 Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D8H1

Cat no : CL594-67733

Synonyms

MTA70, MT A70, Methyltransferase-like protein 3, hMETTL3, EC:2.1.1.348

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Validation Data Gallery

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  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:400.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  IF Staining of mouse testis using CL594-67733

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 METTL3 antibody (CL594-67733, Clone: 2D8H1 ) at dilution of 1:200.

• 

  FC experiment of HEK-293 using CL594-67733

  1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human METTL3 (CL594-67733, Clone:2D8H1) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF-P detected in: mouse testis tissue
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67733 targets METTL3 in IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: METTL3 fusion protein Ag7110
• Full Name: methyltransferase like 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC001650
• Gene Symbol: METTL3
• Gene ID (NCBI): 56339
• RRID: AB_2920176
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.