MEPE Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MEPE Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18804-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 18804-1-AP (same clone as 18804-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 18804-1-AP (same clone as 18804-1-PBS)

  WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 18804-1-AP (same clone as 18804-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 18804-1-AP (same clone as 18804-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 18804-1-AP (same clone as 18804-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A2780 using 18804-1-AP (same clone as 18804-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18804-1-PBS in a different storage buffer formulation.

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Product Information

18804-1-PBS targets MEPE in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: matrix extracellular phosphoglycoprotein
• Calculated Molecular Weight: 525 aa, 58 kDa
• Observed Molecular Weight: 43 kDa, 98 kDa
• GenBank Accession Number: BC128158
• Gene Symbol: MEPE
• Gene ID (NCBI): 56955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).