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MEPE Polyclonal antibody

MEPE Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18804-1-AP

Synonyms

MEPE, OF45, Osteoblast/osteocyte factor 45

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of HepG2 using 18804-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of HeLa using 18804-1-AP

    WB result of MEPE antibody (18804-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MEPE transfected HeLa cells.

  • Western Blot (WB) analysis of A375 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of A375 using 18804-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of MCF-7 using 18804-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of HeLa using 18804-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using MEPE Polyclonal antibody (18804-1-AP)

    WB analysis of A2780 using 18804-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 18804-1-AP (MEPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, A2780 cells, A375 cells, HeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18804-1-AP targets MEPE in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name matrix extracellular phosphoglycoprotein
Calculated Molecular Weight 525 aa, 58 kDa
Observed Molecular Weight 43 kDa, 98 kDa
GenBank Accession NumberBC128158
Gene Symbol MEPE
Gene ID (NCBI) 56955
RRIDAB_2143574
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Matrix extracellular phosphoglycoprotein (MEPE), also known as osteoblast/osteocyte factor 45 (OF45), is amember of the small integrin-binding ligand N-linked glycopro-tein (SIBLING) family (PMID: 12952171). MEPE promotes renal phosphate excretion and modulates mineralization (PMID: 22582013). It can be phosphorylated by secreted protein kinases (PMID: 14962809). MEPE is expressed in osteoblasts, osteocytes and odontoblasts, and the expression has also been reported in numerous human tumor cell lines (PMID: 14962809; 20145617). It is a 525-amino acid protein with a calculated molecular weight of 58 kDa. This antibody raised against a synthetic peptide (CTHGRKYHYVPHRQNNSTR) detects a major band (43 kDa) and two minor bands (98 kDa and 34 kDa).

Protocols

Product Specific Protocols
WB protocol for MEPE antibody 18804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols