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MELK Recombinant antibody, PBS Only (Detector)

MELK Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230106E8

Cat no : 82893-6-PBS

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00075-4

    Sandwich ELISA standard curve of MP00075-4

    Sandwich ELISA standard curve of MP00075-4, Human MELK Recombinant Matched Antibody Pair - PBS only. 82893-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1897. 82893-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • Sample test of MP00075-4

    Sample test of MP00075-4

    NCCIT and K-562 cell lysate were measured. The human MELK concentration of detected samples was determined to be 15.9 ng/mL (based on a 4.5 mg/mL extract load) in NCCIT cell lysate and 16.4 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate.

Product Information

82893-6-PBS targets MELK as part of a matched antibody pair:

MP00075-4: 82893-2-PBS capture and 82893-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MELK fusion protein Ag1897
Full Name maternal embryonic leucine zipper kinase
Calculated Molecular Weight 651 aa, 75 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.