MELK Recombinant antibody, PBS Only (Capture/Detector)

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  WB analysis using 82893-2-RR (same clone as 82893-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82893-2-RR (MELK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00075-4

  Sandwich ELISA standard curve of MP00075-4, Human MELK Recombinant Matched Antibody Pair - PBS only. 82893-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1897. 82893-6-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Sample test of MP00075-4

  NCCIT and K-562 cell lysate were measured. The human MELK concentration of detected samples was determined to be 15.9 ng/mL (based on a 4.5 mg/mL extract load) in NCCIT cell lysate and 16.4 ng/mL (based on a 4.5 mg/mL extract load) in K-562 cell lysate.

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  IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 82893-2-RR (same clone as 82893-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82893-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00075-1

  Cytometric bead array standard curve of MP00075-1, MELK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82893-2-PBS. Detection antibody: 82893-3-PBS. Standard: Ag1897. Range: 0.313-20 ng/mL.

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  Cytometric bead array standard curve of MP00075-2

  Cytometric bead array standard curve of MP00075-2, MELK Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82893-4-PBS. Detection antibody: 82893-2-PBS. Standard: Ag1897. Range: 0.313-20 ng/mL.

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  Affinity and Kinetic Characterization of 82893-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82893-2-RR against Human MELK were performed. The affinity constant is below 1 pM.

MELK Uni-rAb^TM Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 82893-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

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82893-2-RR

Product Information

82893-2-PBS targets MELK as part of a matched antibody pair:

MP00075-1: 82893-2-PBS capture and 82893-3-PBS detection (validated in Cytometric bead array)

MP00075-2: 82893-4-PBS capture and 82893-2-PBS detection (validated in Cytometric bead array)

MP00075-4: 82893-2-PBS capture and 82893-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MELK fusion protein Ag1897
• Full Name: maternal embryonic leucine zipper kinase
• Calculated Molecular Weight: 651 aa, 75 kDa
• Observed Molecular Weight: 50-70 kDa
• GenBank Accession Number: BC014039
• Gene Symbol: MELK
• Gene ID (NCBI): 9833
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14680
• Storage Buffer: 100% PBS pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.