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MELK Recombinant antibody

MELK Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230106A4

Cat no : 82893-2-RR

Synonyms

KIAA0175, hPK38, hMELK, EC:2.7.11.1, EC:2.7.10.2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MELK Recombinant antibody (82893-2-RR)

    WB analysis using 82893-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82893-2-RR (MELK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MELK Recombinant antibody (82893-2-RR)

    IHC staining of mouse kidney using 82893-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using MELK Recombinant antibody (82893-2-RR)

    IHC staining of mouse kidney using 82893-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82893-2-RR (MELK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82893-2-RR

    Affinity and Kinetic Characterization of 82893-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82893-2-RR against Human MELK were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inNCCIT cells, HepG2 cells, MCF-7 cells, MDA-MB-231 cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82893-2-RR targets MELK in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MELK fusion protein Ag1897
Full Name maternal embryonic leucine zipper kinase
Calculated Molecular Weight 651 aa, 75 kDa
Observed Molecular Weight50-70 kDa
GenBank Accession NumberBC014039
Gene Symbol MELK
Gene ID (NCBI) 9833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MELK is involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. It also plays a key role in cell proliferation and carcinogenesis, and it is required for proliferation of embryonic and postnatal multipotent neural progenitors.

Protocols

Product Specific Protocols
WB protocol for MELK antibody 82893-2-RRDownload protocol
IHC protocol for MELK antibody 82893-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols