MEK1/2 Monoclonal antibody, PBS Only

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  WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67410-1-Ig (same clone as 67410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67410-1-Ig (same clone as 67410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MEK1/2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67410-1-Ig (same clone as 67410-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MEK1/2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67410-1-PBS in a different storage buffer formulation.

MEK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67410-1-PBS

Clone No.1H8C2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MAP2K2, MEK2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2

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Product Information

67410-1-PBS targets MEK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MEK1/2 fusion protein Ag29871
• Full Name: mitogen-activated protein kinase kinase 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-47 kDa
• GenBank Accession Number: BC018645
• Gene Symbol: MEK2
• Gene ID (NCBI): 5605
• RRID: AB_2882651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P36507
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 67410-1-Ig can recognize both the MEK2 and MEK1.