MEK2 Monoclonal antibody

MEK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

CloneNo.

1H8C2

Cat no : 67410-1-Ig

Synonyms

MAP2K2, MAP kinase kinase 2, ERK activator kinase 2, EC:2.7.12.2, Dual specificity mitogen-activated protein kinase kinase 2

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Validation Data Gallery

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  WB analysis using 67410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MAP2K2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MEK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67410-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67410-1-Ig (MAP2K2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MAP2K2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67410-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67410-1-Ig (MAP2K2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67410-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MAP2K2 antibody (67410-1-Ig, Clone: 1H8C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells, Neuro-2a cells, MOLT-4 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

67410-1-Ig targets MEK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MEK2 fusion protein Ag29871
• Full Name: mitogen-activated protein kinase kinase 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-47 kDa
• GenBank Accession Number: BC018645
• Gene Symbol: MEK2
• Gene ID (NCBI): 5605
• RRID: AB_2882651
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

Publications

Species	Application	Title
mouse	IHC	Front Immunol; Development and validation of cuproptosis-related lncRNAs associated with pancreatic cancer immune microenvironment based on single-cell; Authors - Yimeng Sun
mouse	WB	Mol Cell Biochem; Anti-inflammatory effects of extracellular vesicles from Morchella on LPS-stimulated RAW264.7 cells via the ROS-mediated p38 MAPK signaling pathway.; Authors - Qi Chen
human	WB	PNAS Nexus; A potent tumor-selective ERK pathway inactivator with high therapeutic index.; Authors - Zehua Zuo
human	WB	Signal Transduct Target Ther; IGF-1R inhibition induces MEK phosphorylation to promote survival in colon carcinomas.; Authors - Qing Wang
mouse	WB	Proc Natl Acad Sci U S A; ERK and c-Myc signaling in host-derived tumor endothelial cells is essential for solid tumor growth; Authors - Zehua Zuo
human	WB	Front Med; 5'-tiRNA-Gln inhibits hepatocellular carcinoma progression by repressing translation through the interaction with eukaryotic initiation factor 4A-I; Authors - Chengdong Wu