MEK1/2 Polyclonal antibody, PBS Only

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  WB analysis using 11049-1-AP (same clone as 11049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11049-1-AP (same clone as 11049-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11049-1-AP (same clone as 11049-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11049-1-AP (MEK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

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  IP experiment of HL-60 using 11049-1-AP (same clone as 11049-1-PBS)

  IP result of anti-MEK1/2 (IP:11049-1-AP, 4ug; Detection:11049-1-AP 1:1000) with HL-60 cells lysate 1280ug. This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11049-1-AP (same clone as 11049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11049-1-AP (same clone as 11049-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11049-1-AP (MEK1/2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 11049-1-AP (same clone as 11049-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using MEK1/2 antibody (11049-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 11049-1-AP (same clone as 11049-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 11049-1-AP (MEK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11049-1-PBS in a different storage buffer formulation.

MEK1/2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11049-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

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Product Information

11049-1-PBS targets MEK1/2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEK1/2 fusion protein Ag1481
• Full Name: mitogen-activated protein kinase kinase 2
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44-47 kDa
• GenBank Accession Number: BC018645
• Gene Symbol: MEK2
• Gene ID (NCBI): 5605
• RRID: AB_2140649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36507
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway. 11049-1-AP can recognize both the MEK2 and MEK1.