MEFV Polyclonal antibody, PBS Only

MEFV Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24280-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human plasma using 24280-1-AP (same clone as 24280-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24280-1-PBS in a different storage buffer formulation.

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  WB analysis using 24280-1-AP (same clone as 24280-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 24280-1-AP (MEFV antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24280-1-PBS in a different storage buffer formulation.

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  IP experiment of THP-1 using 24280-1-AP (same clone as 24280-1-PBS)

  IP result of anti-MEFV (IP:24280-1-AP, 4ug; Detection:24280-1-AP 1:5000) with THP-1 cells lysate 1820 ug. This data was developed using the same antibody clone with 24280-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24280-1-AP (same clone as 24280-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MEFV antibody (24280-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 24280-1-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 24280-1-AP (same clone as 24280-1-PBS)

  1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human MEFV (24280-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 24280-1-PBS in a different storage buffer formulation.

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Product Information

24280-1-PBS targets MEFV in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEFV fusion protein Ag18000
• Full Name: Mediterranean fever
• Calculated Molecular Weight: 781 aa, 86 kDa
• Observed Molecular Weight: 86 kDa
• GenBank Accession Number: BC101511
• Gene Symbol: MEFV
• Gene ID (NCBI): 4210
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MEFV, a gene encoding a protein of unknown function, is the genetic cause of Familial Mediterranean fever (FMF), a recessively inherited disorder characterized by recurring attacks of fever and serositis. Recently mutation of MEFV has been reported to cause autoinflammatory disease distinct from FMF.