CoraLite® Plus 488-conjugated MED4 Monoclonal antibody

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  FC experiment of HepG2 using CL488-67839

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MED4 (CL488-67839, Clone:2E6F4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MED4 Monoclonal Antibody for FC (Intra)

Cat No. CL488-67839

Clone No.2E6F4

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, rat

Applications

FC (Intra)

Synonyms

MED4, Mediator of RNA polymerase II transcription subunit 4, TRAP36, VDRIP, Vitamin D3 receptor-interacting protein complex 36 kDa component, 2E6F4, Activator-recruited cofactor 36 kDa component, ARC36, DRIP36, HSPC126

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Tested Applications

• Positive FC (Intra) detected in: HepG2 cells
• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67839 targets MED4 in FC (Intra) applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MED4 fusion protein Ag8562
• Full Name: mediator complex subunit 4
• Calculated Molecular Weight: 270 aa, 30 kDa
• Observed Molecular Weight: ~36 kDa
• GenBank Accession Number: BC005189
• Gene Symbol: MED4
• Gene ID (NCBI): 29079
• RRID: AB_2934573
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NPJ6
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MED4 is a component of the Meditor complex, which is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Meditor functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. It also have an alternative name called "Activator-recruited cofactor 36 kDa component (ARC36) ".