MED15 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MED15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11566-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SMMC-7721 using 11566-1-AP (same clone as 11566-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

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  WB analysis of HuH-7 using 11566-1-AP (same clone as 11566-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 11566-1-AP (same clone as 11566-1-PBS)

  IP result of anti-MED15 (IP:11566-1-AP, 3ug; Detection:11566-1-AP 1:500) with K-562 cells lysate 3440ug. This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 11566-1-AP (same clone as 11566-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11566-1-AP (MED15 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 11566-1-AP (same clone as 11566-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 11566-1-AP (MED15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11566-1-PBS in a different storage buffer formulation.

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Product Information

11566-1-PBS targets MED15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED15 fusion protein Ag2143
• Full Name: mediator complex subunit 15
• Calculated Molecular Weight: 788 aa, 87 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC017110
• Gene Symbol: MED15
• Gene ID (NCBI): 51586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).