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MED15 Polyclonal antibody
MED15 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 11566-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, SMMC-7721 cells, HuH-7 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 10 publications below |
| IHC | See 7 publications below |
| IF | See 6 publications below |
| ChIP | See 4 publications below |
Product Information
11566-1-AP targets MED15 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MED15 fusion protein Ag2143 |
| Full Name | mediator complex subunit 15 |
| Calculated Molecular Weight | 788 aa, 87 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC017110 |
| Gene Symbol | MED15 |
| Gene ID (NCBI) | 51586 |
| RRID | AB_2141975 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED15 antibody 11566-1-AP | Download protocol |
| IHC protocol for MED15 antibody 11566-1-AP | Download protocol |
| IF protocol for MED15 antibody 11566-1-AP | Download protocol |
| IP protocol for MED15 antibody 11566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. | ||
Cell Rep Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF. | ||
Int J Cancer MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.
| ||
PLoS Genet Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans. | ||
J Biol Chem Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune-response genes. | ||
Am J Pathol Clinical and Molecular Implications of MED15 in Head and Neck Squamous Cell Carcinoma. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH SITING (Verified Customer) (04-02-2021) | between 75-100
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