Validation Data Gallery
|Positive WB detected in||MDA-MB-453s cells, human brain tissue, MCF-7 cells, mouse heart tissue, mouse lung tissue, SH-SY5Y cells, mouse ovary tissue, mouse brain tissue, rat lung tissue|
|Positive IP detected in||MCF-7 cells, HEK-293T cells|
|Positive IHC detected in||human brain tissue, rat brain tissue, human gliomas tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, mouse cerebellum tissue|
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10861-1-AP targets MECP2 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MECP2 fusion protein Ag1309|
|Full Name||methyl CpG binding protein 2 (Rett syndrome)|
|Calculated molecular weight||52 kDa, 53 kDa|
|Observed molecular weight||75 kDa|
|GenBank accession number||BC011612|
|Gene ID (NCBI)||4204|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
METHYL-CpG-BINDING PROTEIN 2(MECP2), is a chromatin-associated protein that can both activate and repress transcription. Mecp2 in the adult mouse is high in the brain, lung, and spleen, lower in heart and kidney. MECP2 takes part in the control of neuronal activity-dependent gene regulation, and this process may underlie the pathology of Rett syndrome, a severe developmental disorder with autistic phenotypes. As compared to wild-type monkeys, MECP2 transgenic monkeys exhibited a higher frequency of repetitive circular locomotion and increased stress responses, as measured by the threat-related anxiety and defensive test. Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Two isoforms of MECP2 exist due to alternative splicing events.This antibody reacts with the MECP2 and phosphorylated MECP2 proteins. The calculated molecular weight of MECP2 is a 52 kDa, but the post-modified MECP2 protein is about 75-80 kDa (PMID: 12160743).
|Product Specific Protocols|
|WB protocol for MECP2 antibody 10861-1-AP||Download protocol|
|IHC protocol for MECP2 antibody 10861-1-AP||Download protocol|
|IF protocol for MECP2 antibody 10861-1-AP||Download protocol|
|IP protocol for MECP2 antibody 10861-1-AP||Download protocol|
|Click here to view our Standard Protocols|
MeCP2 is a microsatellite binding protein that protects CA repeats from nucleosome invasion.
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Specificity of Antinuclear Autoantibodies Recognizing the Dense Fine Speckled Nuclear Pattern: Preferential Targeting of DFS70/LEDGFp75 Over its Interacting Partner MeCP2.
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