MDM1 Recombinant antibody, PBS Only

MDM1 Uni-rAb^TM Recombinant Antibody for FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230425E2

Cat no : 83141-5-PBS

Synonyms

Nuclear protein MDM1, 230425E2

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Validation Data Gallery

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  FC experiment of A431 using 83141-5-RR (same clone as 83141-5-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Mdm1 Recombinant Antibody (83141-5-RR, Clone:230425E2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83141-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83141-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83141-5-RR against Human MDM1 were performed. The affinity constant is 0.563 nM.

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Product Information

The immunogen of 83141-5-PBS is MDM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MDM1 fusion protein Ag10541
• Full Name: Mdm1 nuclear protein homolog (mouse)
• Calculated Molecular Weight: 81 kDa
• GenBank Accession Number: BC028355
• Gene Symbol: MDM1
• Gene ID (NCBI): 56890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Mdm1 is highly conserved in mammals as four sorting nexins (Snx): Snx13, 14, 19, and 25. Snx14 alleles are implicated in an autosomal-recessive neurological disease characterized by cellular vacuolization and granular plaque accumulation in late endosomes or autophagosomes. (PMID: 26283797)