MDH2 Monoclonal antibody

MDH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A9G11

Cat no : 68878-1-Ig

Synonyms

MDH 2, MDH, EC:1.1.1.37, 2A9G11

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Validation Data Gallery

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  WB analysis using 68878-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68878-1-Ig (MDH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68878-1-Ig targets MDH2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MDH2 fusion protein Ag7750
• Full Name: malate dehydrogenase 2, NAD (mitochondrial)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC001917
• Gene Symbol: MDH2
• Gene ID (NCBI): 4191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MDH2 encodes the Krebs cycle enzyme mitochondrial malate dehydrogenase, which catalyzes the reversible oxidation of malate to oxaloacetate and plays a role in the malate-aspartate NADH shuttle. Mutations in MDH2 Cause Developmental and epileptic encephalopathy 51 (PMID: 27989324).