MCT4 Recombinant antibody

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  WB analysis using 84797-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84797-4-RR (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse skeletal muscle using 84797-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using MCT4 antibody (84797-4-RR, Clone: 242296E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84797-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84797-4-RR against Human MCT4 were performed. The affinity constant is 3.02 nM.

MCT4 {ptg:Brand} Recombinant Antibody for WB, IF-P, ELISA

Cat No. 84797-4-RR

Clone No.242296E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Synonyms

SLC16A3, MCT-4, MCT3, MCT 4, 242296E3

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells
• Positive IF-P detected in: mouse skeletal muscle tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84797-4-RR targets MCT4 in WB, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MCT4 fusion protein Ag18788
• Full Name: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• Calculated Molecular Weight: 465 aa, 49 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC112269
• Gene Symbol: MCT4
• Gene ID (NCBI): 9123
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15427
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.