MCM7 Monoclonal antibody, PBS Only

MCM7 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G1F6

Cat no : 67446-1-PBS

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

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Validation Data Gallery

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  WB analysis using 67446-1-Ig (same clone as 67446-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67446-1-Ig (MCM7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 67446-1-Ig (same clone as 67446-1-PBS)

  IP result of anti-MCM7 (IP:67446-1-Ig, 4ug; Detection:67446-1-Ig 1:2000) with HeLa cells lysate 1040 ug. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67446-1-Ig (same clone as 67446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67446-1-Ig (same clone as 67446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67446-1-PBS targets MCM7 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MCM7 fusion protein Ag28558
• Full Name: minichromosome maintenance complex component 7
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC013375
• Gene Symbol: MCM7
• Gene ID (NCBI): 4176
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].