MCM7 antibody (67446-1-PBS)

Filter: AllWBIPIHC

WB analysis using 67446-1-Ig (same clone as 67446-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67446-1-Ig (MCM7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 67446-1-Ig (same clone as 67446-1-PBS)
IP result of anti-MCM7 (IP:67446-1-Ig, 4ug; Detection:67446-1-Ig 1:2000) with HeLa cells lysate 1040 ug. This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67446-1-Ig (same clone as 67446-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67446-1-Ig (same clone as 67446-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67446-1-Ig (same clone as 67446-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67446-1-Ig (MCM7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67446-1-PBS in a different storage buffer formulation.

MCM7 Monoclonal antibody, PBS Only

MCM7 Monoclonal Antibody for WB, IP, IHC, Indirect ELISA

Cat No. 67446-1-PBS

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Synonyms

CDABP0042, CDC47, CDC47 homolog, MCM2, MCM7, P1.1 MCM3, P1CDC47, P85MCM, PNAS 146

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

67446-1-PBS targets MCM7 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	MCM7 fusion protein Ag28558 Predict reactive species
Full Name	minichromosome maintenance complex component 7
Calculated Molecular Weight	81 kDa
Observed Molecular Weight	85 kDa
GenBank Accession Number	BC013375
Gene Symbol	MCM7
Gene ID (NCBI)	4176
RRID	AB_2882680
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P33993
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

MCM7, also named as CDC47 homolog or P1.1-MCM3, is a 719 amino acid protein, which belongs to the MCM family. MCM7 acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. the MCM2–7 complex participates in the pre-replication complex formation and exhibits helicase activity which makes DNA unwind, and resulting in recruitment of DNA polymerases and initiation of DNA replication and elongation [PMID: 29463213 ]. An immunohistochemical study has shown that MCM7 is increased in HCC. Silencing of MCM7 with shRNA inhibits the malignant behavior of HCC cells via cell cycle arrest and apoptosis [[PMID: 29463213].