MCM2 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 84511-2-RR (same clone as 84511-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84511-2-RR (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01392-2

  Sandwich ELISA standard curve of MP01392-2, Human MCM2 Recombinant Matched Antibody Pair - PBS only. 84511-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0798. 84511-3-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Sample test of MP01392-2

  Serum of six individual healthy human donors was measured. The MCM2 concentration of detected samples was determined to be 2.29 ng/mL with a range of ND-8.68 ng/mL.

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  Sample test of MP01392-2

  The mean MCM2 concentration was determined to be 58.85 ng/mL in A549 cell extract based on a 2.70 mg/mL extract load and 56.17 ng/mL in HEK-293 cell extract based on a 3.30 mg/mL extract load.

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  IHC staining of human tonsillitis using 84511-2-RR (same clone as 84511-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84511-2-RR (MCM2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84511-2-RR (same clone as 84511-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84511-2-RR (MCM2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84511-2-RR (same clone as 84511-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MCM2 antibody (84511-2-RR, Clone: 241968D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84511-2-RR (same clone as 84511-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (84511-2-RR, Clone: 241968D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84511-2-RR (same clone as 84511-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug MCM2 Recombinant antibody (84511-2-RR, Clone:241968D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 84511-2-RR (same clone as 84511-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug MCM2 Recombinant antibody (84511-2-RR, Clone:241968D3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84511-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01392-1

  Cytometric bead array standard curve of MP01392-1, MCM2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84511-2-PBS. Detection antibody: 84511-1-PBS. Standard: Ag0798. Range: 0.781-100 ng/mL.

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  Cytometric bead array standard curve of MP01392-2

  Cytometric bead array standard curve of MP01392-2, MCM2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84511-2-PBS. Detection antibody: 84511-3-PBS. Standard: Ag0798. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84511-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84511-2-RR against Human MCM2 were performed. The affinity constant is 0.453 nM.

MCM2 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84511-2-PBS

Clone No.241968D3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

KIAA0030, EC:3.6.4.12, CDCL1, CCNL1, BM28

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Product Information

84511-2-PBS targets MCM2 as part of a matched antibody pair:

MP01392-1: 84511-2-PBS capture and 84511-1-PBS detection (validated in Cytometric bead array)

MP01392-2: 84511-2-PBS capture and 84511-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MCM2 fusion protein Ag0798
• Full Name: minichromosome maintenance complex component 2
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 116-125 kDa
• GenBank Accession Number: BC007670
• Gene Symbol: MCM2
• Gene ID (NCBI): 4171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49736
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.