MCM2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MCM2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F4D10

Cat no : 66204-1-PBS

Synonyms

BM28, CCNL1, cdc19, CDCL1, D3S3194, KIAA0030, MCM2, MITOTIN, Nuclear protein BM28

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Validation Data Gallery

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  WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

  WB result of MCM2 antibody (66204-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66204-1-Ig (same clone as 66204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 66204-1-Ig (same clone as 66204-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66204-1-Ig (same clone as 66204-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66204-1-Ig (same clone as 66204-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66204-1-Ig (same clone as 66204-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66204-1-Ig (MCM2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66204-1-Ig (MCM2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66204-1-Ig (same clone as 66204-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCM2 antibody (66204-1-Ig, Clone: 1F4D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66204-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66204-1-PBS targets MCM2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MCM2 fusion protein Ag0798
• Full Name: minichromosome maintenance complex component 2
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 120-125 kDa
• GenBank Accession Number: BC007670
• Gene Symbol: MCM2
• Gene ID (NCBI): 4171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.