MCL1 Recombinant antibody, PBS Only

MCL1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5P13

Cat no : 82695-4-PBS

Synonyms

Induced myeloid leukemia cell differentiation protein Mcl-1, EAT, Bcl-2-related protein EAT/mcl1, Bcl-2-like protein 3, Bcl2-L-3

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Validation Data Gallery

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  WB analysis using 82695-4-RR (same clone as 82695-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82695-4-RR (MCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-4-RR (MCL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82695-4-RR (MCL1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-4-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovarian cancer using 82695-4-RR (same clone as 82695-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 82695-4-RR (MCL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 82695-4-RR (same clone as 82695-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MCL1 antibody (82695-4-RR, Clone: 5P13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  FC experiment of Ramos using 82695-4-RR (same clone as 82695-4-PBS)

  1x10^6 Ramos cells were intracellularly stained with 0.25 ug MCL1 Recombinant antibody (82695-4-RR, Clone:5P13) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82695-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82695-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82695-4-RR against Human MCL1 were performed. The affinity constant is 2.13 nM.

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Product Information

82695-4-PBS targets MCL1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MCL1 fusion protein Ag10397
• Full Name: myeloid cell leukemia sequence 1 (BCL2-related)
• Calculated Molecular Weight: 350 aa, 37 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC107735
• Gene Symbol: MCL1
• Gene ID (NCBI): 4170
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MCL-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line. Similar to BCL2 and BCL2L1, MCL1 can interact with BAX and/or BAK1 to inhibit mitochondria-mediated apoptosis. Mcl-1 is critical for the proliferation and survival of myeloma cells in vitro, and overexpression of Mcl-1 protein in myeloma cells is associated with relapse and short event-free survival in multiple myeloma patients. Recent studies show that MCL-1 is upregulated in numerous haematological and solid tumor malignancies. Therefore, MCL-1 has been suggested as a potential new therapeutic target. MCL-1 can be alternatively spliced into a long form (MCL-1L, 40 kDa) or a short form (MCL-1S, 30 kDa). (PMID: 15467463, PMID: 15147382)