IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
IF Staining of HuH-7 using 83546-3-RR (same clone as 83546-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
IF Staining of HuH-7 using 83546-3-PBS
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-PBS, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-PBS, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC experiment of A549 using 83546-3-RR (same clone as 83546-3-PBS)
1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 83546-3-RR
Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.
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MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.
IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 83546-3-RR (same clone as 83546-3-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83546-3-RR (MBOAT7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HuH-7 using 83546-3-RR (same clone as 83546-3-PBS)
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
IF Staining of HuH-7 using 83546-3-PBS
Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-PBS, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
FC (INTRA) Figures
FC experiment of A549 using 83546-3-RR (same clone as 83546-3-PBS)
1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83546-3-PBS in a different storage buffer formulation.
AFFINITY Figures
Affinity and Kinetic Characterization of 83546-3-RR
Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
MBOAT7 Recombinant antibody
Catalog Number
83546-3-PBS
Citations
-
Dilutions
IF/ICC : 1:125-1:500
Applications
IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
