MBOAT7 Recombinant antibody

MBOAT7 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240505E8

Cat no : 83546-3-RR

Synonyms

h mboa 7, EC:2.3.1.-, Bladder and breast carcinoma-overexpressed gene 1 protein, BB1, 240505E8

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Validation Data Gallery

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  IF Staining of HuH-7 using 83546-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using MBOAT7 antibody (83546-3-RR, Clone: 240505E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  FC experiment of A549 using 83546-3-RR

  1x10^6 A549 cells were intracellularly stained with 0.25 ug MBOAT7 Recombinant antibody (83546-3-RR, Clone:240505E8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83546-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83546-3-RR against Human MBOAT7 were performed. The affinity constant is 0.573 nM.

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Tested Applications

• Positive IF/ICC detected in: HuH-7 cells
• Positive FC (Intra) detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83546-3-RR is MBOAT7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MBOAT7 fusion protein Ag24466
• Full Name: membrane bound O-acyltransferase domain containing 7
• Calculated Molecular Weight: 472 aa, 53 kDa
• GenBank Accession Number: BC006309
• Gene Symbol: MBOAT7
• Gene ID (NCBI): 79143
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBOAT7 (membrane-bound O-acyltransferase domain containing 7), also known as LPIAT, LENG4, or BB1, belongs to the MBOAT superfamily. MBOAT7 acylates lyso-phosphatidylinositol (lyso-PI) with arachidonyl-CoA and is involved in phosphatidylinositol acyl-chain remodeling in the Lands cycle (PMID: 30959108; PMID: 35509994). MBOAT7 deficiency in humans leads to developmental disorders of the central nervous system, with intellectual disability, epilepsy, and autism spectrum disorder (PMID: 37316513). The calculated molecular weight of MBOAT7 is 53 kDa. This antibody can recognize the 45 kDa isoform of MBOAT7.