Biotin-conjugated MAdCAM1 Monoclonal antibody

MAdCAM1 Monoclonal Antibody for IHC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IHC

Conjugate

Biotin

CloneNo.

2A12E8

Cat no : Biotin-66594

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1

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Validation Data Gallery

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  IHC staining of human small intestine using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using Biotin-66594

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-66594 (MAdCAM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human small intestine tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-66594 targets MAdCAM1 in IHC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAdCAM1 fusion protein Ag16184
• Full Name: mucosal vascular addressin cell adhesion molecule 1
• Calculated Molecular Weight: 406 aa, 43 kDa
• GenBank Accession Number: BC142629
• Gene Symbol: MADCAM1
• Gene ID (NCBI): 8174
• RRID: AB_2918948
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.