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MAX Recombinant antibody, PBS Only

MAX Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230215E3

Cat no : 82958-1-PBS

Synonyms

bHLHd8, bHLHd7, bHLHd6, bHLHd5, bHLHd4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAX Recombinant antibody (82958-1-RR)

    WB analysis using 82958-1-RR (same clone as 82958-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82958-1-RR (MAX antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82958-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82958-1-RR

    Affinity and Kinetic Characterization of 82958-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82958-1-RR against Human MAX were performed. The affinity constant is 0.257 nM.

Product Information

82958-1-PBS targets MAX in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAX fusion protein Ag0680
Full Name MYC associated factor X
Calculated Molecular Weight 18 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC003525
Gene Symbol MAX
Gene ID (NCBI) 4149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.