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MAX Recombinant antibody

MAX Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230215E3

Cat no : 82958-1-RR

Synonyms

bHLHd8, bHLHd7, bHLHd6, bHLHd5, bHLHd4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAX Recombinant antibody (82958-1-RR)

    WB analysis using 82958-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82958-1-RR (MAX antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82958-1-RR

    Affinity and Kinetic Characterization of 82958-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82958-1-RR against Human MAX were performed. The affinity constant is 0.257 nM.

Tested Applications

Positive WB detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82958-1-RR targets MAX in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAX fusion protein Ag0680
Full Name MYC associated factor X
Calculated Molecular Weight 18 kDa
Observed Molecular Weight22 kDa
GenBank Accession NumberBC003525
Gene Symbol MAX
Gene ID (NCBI) 4149
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.

Protocols

Product Specific Protocols
WB protocol for MAX antibody 82958-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols