MATN1 Polyclonal antibody

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  WB analysis using 28566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28566-1-AP (MATN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 28566-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using MATN1 antibody (28566-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

MATN1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 28566-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

pig, human

Applications

WB, IF/ICC, ELISA

Synonyms

Cartilage matrix protein, CMP, CRTM, MATN1, Matrilin 1

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Tested Applications

• Positive WB detected in: pig cartilage tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28566-1-AP targets MATN1 in WB, IF/ICC, ELISA applications and shows reactivity with pig, human samples.

• Tested Reactivity: pig, human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MATN1 fusion protein Ag29386
• Full Name: matrilin 1, cartilage matrix protein
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_002379
• Gene Symbol: MATN1
• Gene ID (NCBI): 4146
• RRID: AB_3669662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P21941
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MATN1 (matrilin 1), also known as CMP. It is expected to be located in extracellular space, the protein is mainly expressed in cartilage tissue and retina tissue. Also, the protein is a member of von Willebrand factor A domain containing protein family. This family of proteins are thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. Mutations of this gene have been associated with variety of inherited chondrodysplasias. It is reproted that COMP and matrilin-1 elute together in the gel filtration of cartilage extracts and can be co-immunoprecipitated with the presence of calcium (PMID: 15075323). The calculated molecular weight of MATN1 is 54 kDa.