MAPK13 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MAPK13 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B12A6

Cat no : 60252-1-PBS

Synonyms

MAP kinase 13, MAP kinase p38 delta, MAPK 13, MAPK13, p38delta, PRKM13, SAPK4

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Validation Data Gallery

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  WB analysis of Ramos using 60252-1-Ig (same clone as 60252-1-PBS)

  Ramos cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 60252-1-Ig (same clone as 60252-1-PBS)

  WB result of MAPK13 antibody (60252-1-Ig, 1:2000) with si-Control and si-MAPK13 transfected HepG2 cells. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 60252-1-Ig (same clone as 60252-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 60252-1-Ig (same clone as 60252-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60252-1-Ig (MAPK13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60252-1-Ig (same clone as 60252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 60252-1-Ig (same clone as 60252-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60252-1-Ig (MAPK13 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60252-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60252-1-PBS targets MAPK13 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAPK13 fusion protein Ag21240
• Full Name: mitogen-activated protein kinase 13
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC000433
• Gene Symbol: MAPK13
• Gene ID (NCBI): 5603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAPK13(Mitogen-activated protein kinase 13) is also named as PRKM13, SAPK4 and belongs to the protein kinase superfamily. MAPK13 is one of the four p38 MAPKs which play an important role in the cascades of cellular responses evoked by extracellular stimuli such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors such as ELK1 and ATF2.