MAP4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

MAP4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11229-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11229-1-AP (same clone as 11229-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C2C12 using 11229-1-AP (same clone as 11229-1-PBS)

  C2C12 cell were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 11229-1-AP (same clone as 11229-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 11229-1-AP (same clone as 11229-1-PBS)

  IP result of anti-MAP4 (IP:11229-1-AP, 4ug; Detection:11229-1-AP 1:1000) with HEK-293 cells lysate 1200ug. This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 11229-1-AP (same clone as 11229-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11229-1-PBS in a different storage buffer formulation.

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Product Information

11229-1-PBS targets MAP4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP4 fusion protein Ag1741
• Full Name: microtubule-associated protein 4
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 210-240 kDa
• GenBank Accession Number: BC012794
• Gene Symbol: MAP4
• Gene ID (NCBI): 4134
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)