MAP4 Polyclonal antibody

• Featured Product
• KD/KO Validated

MAP4 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

15

Reviews (2)

Cat no : 11229-1-AP

Synonyms

MAP 4, MAP4

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 11229-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11229-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 11229-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C2C12 using 11229-1-AP

  C2C12 cell were subjected to SDS PAGE followed by western blot with 11229-1-AP (MAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11229-1-AP

  IP result of anti-MAP4 (IP:11229-1-AP, 4ug; Detection:11229-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

• 

  IHC staining of human oesophagus cancer using 11229-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 11229-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 11229-1-AP (MAP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, C6 cells, C2C12 cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human oesophagus cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 12 publications below
• IHC: See 6 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

11229-1-AP targets MAP4 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAP4 fusion protein Ag1741
• Full Name: microtubule-associated protein 4
• Calculated Molecular Weight: 121 kDa
• Observed Molecular Weight: 210-240 kDa
• GenBank Accession Number: BC012794
• Gene Symbol: MAP4
• Gene ID (NCBI): 4134
• RRID: AB_2140681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

Publications

Species	Application	Title
human	IHC	Acta Neuropathol; Divergent brain gene expression patterns associate with distinct cell-specific tau neuropathology traits in progressive supranuclear palsy.; Authors - Mariet Allen
human	WB	Brain; Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.; Authors - Janja Božič
human	WB,IF	J Mol Cell Biol; The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability.; Authors - Yuan Wu
human	WB	Oncogene; Microtubule-associated protein 4 is an important regulator of cell invasion/migration and a potential therapeutic target in esophageal squamous cell carcinoma.; Authors - Y-Y Jiang ; KO Validated
mouse	WB	J Innate Immun; Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis; Authors - Xiaogang Luo
human	WB	Front Cell Infect Microbiol; Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6.; Authors - Jie Zan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alice (Verified Customer) (08-09-2022)

Not bad for western blot. A bit faint but had bands in expected places and can see clear data.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Sheep atrial tissue

MAP4 Antibody Western Blot validation (1:1000 dilution) in Sheep atrial tissue (Cat no:11229-1-AP)

FH

CHAO (Verified Customer) (03-31-2022)

Multiple bands may be due to different post-translational modifications

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse Heart

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