CoraLite®594-conjugated MAP2K3 Recombinant antibody

MAP2K3 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3O10

Cat no : CL594-80137

Synonyms

MEK3, MAPKK 3, MAPK/ERK kinase 3, MAP kinase kinase 3, EC:2.7.12.2

---

Validation Data Gallery

• 

  IF Staining of HeLa using CL594-80137

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 MAP2K3 antibody (CL594-80137, Clone: 3O10 ) at dilution of 1:200.

• 

  FC experiment of HeLa using CL594-80137

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2K3 (CL594-80137, Clone:3O10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of NIH/3T3 using CL594-80137

  1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human MAP2K3 (CL594-80137, Clone:3O10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells, NIH/3T3 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-80137 targets MAP2K3 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase kinase 3
• Calculated Molecular Weight: 347 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC032478
• Gene Symbol: MAP2K3
• Gene ID (NCBI): 5606
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAP2K3, also named as MKK3, is a member of dual specificity kinase group and serves as a specific activator of p38 MAPK with MKK6. MKK3 can be phosphorylated by cytokines and environmental stress at sites Ser189 and Thr193 by MKKK proteins (MEKK 1-4). MKK3 plays a key role in cell differentiation, motility, division and death. It has been reported that MKK3 targeting may represent an effective and potentially safe therapeutic strategy to selectively kill cancer cells in colorectal cancer patients. (PMID: 31695024, 30770795, 26805700, 8622669)