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MAP2 Recombinant antibody, PBS Only

MAP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241653H8

Cat no : 84306-4-PBS

Synonyms

MAP2C, MAP2B, MAP2A, MAP-2, MAP 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAP2 Recombinant antibody (84306-4-RR)

    WB analysis using 84306-4-RR (same clone as 84306-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84306-4-RR (MAP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using MAP2 Recombinant antibody (84306-4-RR)

    IF Staining of SH-SY5Y using 84306-4-RR (same clone as 84306-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using MAP2 antibody (84306-4-RR, Clone: 241653H8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84306-4-PBS in a different storage buffer formulation.

Product Information

84306-4-PBS targets MAP2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAP2 fusion protein Ag11580
Full Name microtubule-associated protein 2
Calculated Molecular Weight 200 kDa
Observed Molecular Weight280-300 kDa, 70-85 kDa
GenBank Accession NumberBC038857
Gene Symbol MAP2
Gene ID (NCBI) 4133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAP2 (microtubule-associated protein 2) is a cytoskeleton protein abundant in the brain and has an important role in neuronal morphogenesis. Multiple high MW and low MW MAP2 isoforms are expressed within the proximal segment of axons, dendrites, and cell bodies. The expression of MAP2 is regulated in both a tissue- and developmentally-specific manner. The 280 kDa MAP2B is present throughout rat brain development, and the slightly larger MAP2A appears first during the end of the second week of postnatal life. MAP2C, composed of several bands of about 70 kDa, is present during early brain development and largely disappears from the mature brain except for the retina, olfactory bulb, and cerebellum. In addition, some isoforms with lower MW around 50-60 kDa also exist. MAP2 antibodies have been widely used to mark the neuron or dendrite formation.