MAP1B Monoclonal antibody

MAP1B Monoclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1E6B9

Cat no : 67423-1-Ig

Synonyms

DKFZp686E1099, DKFZp686F1345, FLJ38954, FUTSCH, MAP 1B, MAP1B, MAP5

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Validation Data Gallery

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  IHC staining of rat brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67423-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67423-1-Ig (MAP1B antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 67423-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 67423-1-Ig (MAP1B antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IHC detected in: rat brain tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

67423-1-Ig targets MAP1B in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Cited Reactivity: rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAP1B fusion protein Ag17709
• Full Name: microtubule-associated protein 1B
• Calculated Molecular Weight: 2468 aa, 271 kDa
• GenBank Accession Number: BC141853
• Gene Symbol: MAP1B
• Gene ID (NCBI): 4131
• RRID: AB_2918490
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microtubule-associated protein 1B (MAP1B) is a cytoskeleton protein which can promote microtubule assembly. Previous reports have suggested that this protein is closely involved in neuronal development based on its extensive expression in the developing brain and moderate in mature neurons. Gene disruption or knockout studies of the MAP1B gene led to a delayed development of the nervous system in mice. It includes the N-terminal heavy chain and a C-terminal light chain. The MAP1B heavy chain has a microtubule-stabilization effect, and contains an actin-binding site that may play a role in the crosslinking of actin and microtubules, a function that may be important in neurite elongation. Various isoforms around 300-350 kDa of MAP1B can be observed due to the differences in phosphorylation state. (10704485)

Publications

Species	Application	Title
rat	WB,IF	Biochem Biophys Res Commun; NWD1 influences the extension of neuronal axons by regulating microtubule stability; Authors - Tiancheng Bao