MAGI2 Recombinant antibody

MAGI2 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240512C10

Cat no : 83561-6-RR

Synonyms

AIP-1, AIP1, AIP 1, ACVRIP1, ACVRINP1

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Validation Data Gallery

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  WB analysis of rat brain using 83561-6-RR

  rat brain tissue were subjected to SDS PAGE followed by western blot with 83561-6-RR (MAGI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83561-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83561-6-RR against Human MAGI2 were performed. The affinity constant is 1.90 nM.

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Tested Applications

• Positive WB detected in: rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83561-6-RR is MAGI2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAGI2 fusion protein Ag18279
• Full Name: membrane associated guanylate kinase, WW and PDZ domain containing 2
• Calculated Molecular Weight: 1455 aa, 159 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC150277
• Gene Symbol: MAGI2
• Gene ID (NCBI): 9863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGI proteins are scaffolding proteins, belonging to the Membrane-Associated Guanylate Kinase Inverted proteins of the MAGUK family. There are three members of the MAGI subfamily, MAGI-1, MAGI-2, and MAGI-3. They are comprised of 6 PDZ domains, 2 WW domains, and 1 GUK domain. They have been proven to mediate the transport and signal transduction of various G protein-coupled receptors (GPCRs) (PMID: 29625175). The antibody is specific to MAGI2.