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MAGEA4 Recombinant antibody, PBS Only (Capture)

MAGEA4 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241558G7

Cat no : 84248-4-PBS

Synonyms

MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4, Cancer/testis antigen 1.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAGEA4 Recombinant antibody (84248-4-RR)

    WB analysis using 84248-4-RR (same clone as 84248-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using MAGEA4 Recombinant antibody (84248-4-RR)

    WB analysis of human testis using 84248-4-RR (same clone as 84248-4-PBS)

    human testis tissue was subjected to SDS PAGE followed by western blot with 84248-4-RR (MAGEA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84248-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01144-1

    Cytometric bead array standard curve of MP01144-1

    Cytometric bead array standard curve of MP01144-1, MAGEA4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84248-4-PBS. Detection antibody: 84248-5-PBS. Standard: Ag3193. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84248-4-RR

    Affinity and Kinetic Characterization of 84248-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84248-4-RR against Human MAGEA4 were performed. The affinity constant is 2.03 nM.

Product Information

84248-4-PBS targets MAGEA4 as part of a matched antibody pair:

MP01144-1: 84248-4-PBS capture and 84248-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGEA4 fusion protein Ag3193
Full Name melanoma antigen family A, 4
Calculated Molecular Weight 317 aa, 35 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC017723
Gene Symbol MAGEA4
Gene ID (NCBI) 4103
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.