MAGEA3 Recombinant antibody

MAGEA3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83480-5-RR
Clone No.240435G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

HIP8, CT1.3, Cancer/testis antigen 1.3, Antigen MZ2-D, Antigen MZ2 D

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA375 cells, Jurkat cells, SGC-7901 cells, NCI-H1299 cells
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83480-5-RR targets MAGEA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MAGEA3 fusion protein Ag0265 Predict reactive species
Full Name melanoma antigen family A, 3
Calculated Molecular Weight 314 aa, 35 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC000340
Gene Symbol MAGEA3
Gene ID (NCBI) 4102
RRIDAB_3671108
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP43357
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.

Protocols

Product Specific Protocols
WB protocol for MAGEA3 antibody 83480-5-RRDownload protocol
IF protocol for MAGEA3 antibody 83480-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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