Tested Applications
Positive WB detected in | A375 cells, Jurkat cells, SGC-7901 cells, NCI-H1299 cells |
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | HeLa cells, Jurkat cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83480-5-RR targets MAGEA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | MAGEA3 fusion protein Ag0265 Predict reactive species |
Full Name | melanoma antigen family A, 3 |
Calculated Molecular Weight | 314 aa, 35 kDa |
Observed Molecular Weight | 45 kDa |
GenBank Accession Number | BC000340 |
Gene Symbol | MAGEA3 |
Gene ID (NCBI) | 4102 |
RRID | AB_3671108 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P43357 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAGEA3 is a member of the MAGEA family, which belongs to chromosome X-clustered cancer/testis antigens. MAGEA3 is expressed in various human cancers including melanoma, brain, breast, lung, thyroid, colorectal, gastric, pancreatic and ovarian cancer. The aberrant expression of MAGEA3 is associated with poor clinical prognosis in many tumor types. The antibody is specific to MAGEA3.
Protocols
Product Specific Protocols | |
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WB protocol for MAGEA3 antibody 83480-5-RR | Download protocol |
IF protocol for MAGEA3 antibody 83480-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |