CoraLite® Plus 647 Anti-Mouse Ly-6C (HK1.4)

Ly-6C Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2c

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

HK1.4

Cat no : CL647-65296

Synonyms

AA682074, AA959465, Ly 6C, Ly6c, Ly6c1

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Validation Data Gallery

• 

  FC experiment of mouse bone marrow cells using CL647-65296

  1x10^6 mouse bone marrow cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse Ly-6C (HK1.4) (CL647-65296, Clone: HK1.4) (red) or 0.25 ug Isotype Control (blue). Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-65296 targets Ly-6C in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: L3 cloned CTL cells
• Full Name: lymphocyte antigen 6 complex, locus C1
• GenBank Accession Number: BC010764
• Gene Symbol: Ly-6C
• Gene ID (NCBI): 17067
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).