LZTFL1 Monoclonal antibody, PBS Only

LZTFL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C1F6

Cat no : 68281-1-PBS

Synonyms

Leucine zipper transcription factor-like protein 1, 2C1F6

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Validation Data Gallery

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  WB analysis using 68281-1-Ig (same clone as 68281-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68281-1-Ig (LZTFL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LZTFL1 (68281-1-Ig, Clone:2C1F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

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Product Information

68281-1-PBS targets LZTFL1 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LZTFL1 fusion protein Ag10733
• Full Name: leucine zipper transcription factor-like 1
• Calculated Molecular Weight: 299 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC025988
• Gene Symbol: LZTFL1
• Gene ID (NCBI): 54585
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)