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LZTFL1 Monoclonal antibody

LZTFL1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2C1F6

Cat no : 68281-1-Ig

Synonyms

Leucine zipper transcription factor-like protein 1, 2C1F6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LZTFL1 Monoclonal antibody (68281-1-Ig)

    WB analysis using 68281-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68281-1-Ig (LZTFL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    IF Staining of Jurkat using 68281-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    FC experiment of Jurkat using 68281-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LZTFL1 (68281-1-Ig, Clone:2C1F6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHCT 116 cells, LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inJurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68281-1-Ig targets LZTFL1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LZTFL1 fusion protein Ag10733
Full Name leucine zipper transcription factor-like 1
Calculated Molecular Weight 299 aa, 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC025988
Gene Symbol LZTFL1
Gene ID (NCBI) 54585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

Protocols

Product Specific Protocols
WB protocol for LZTFL1 antibody 68281-1-IgDownload protocol
IF protocol for LZTFL1 antibody 68281-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols