LYN Monoclonal antibody, PBS Only

LYN Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3C7F2

Cat no : 60211-1-PBS

Synonyms

JTK8, LYN, Tyrosine protein kinase Lyn

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Validation Data Gallery

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  WB analysis of Jurkat using 60211-1-Ig (same clone as 60211-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60211-1-Ig (same clone as 60211-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60211-1-Ig (same clone as 60211-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LYN antibody (60211-1-Ig, Clone: 3C7F2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60211-1-PBS targets LYN in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LYN fusion protein Ag12723
• Full Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Calculated Molecular Weight: 512 aa, 59 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC075002
• Gene Symbol: LYN
• Gene ID (NCBI): 4067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.