LUC7L Recombinant antibody

LUC7L Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240311B10

Cat no : 83585-1-RR

Synonyms

Putative RNA-binding protein Luc7-like 1, LUC7L1, LUC7B1, LUC7 LIKE, hLuc7B1

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Validation Data Gallery

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  WB analysis using 83585-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83585-1-RR (LUC7L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 83585-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 83585-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83585-1-RR (LUC7L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 83585-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LUC7L antibody (83585-1-RR, Clone: 240311B10 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of MCF-7 using 83585-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug LUC7L Recombinant antibody (83585-1-RR, Clone:240311B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83585-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83585-1-RR against Human LUC7L were performed. The affinity constant is 99.9 pM.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, U-251 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83585-1-RR is LUC7L Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LUC7L fusion protein Ag9170
• Full Name: LUC7-like (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38 kDa and 44 kDa
• GenBank Accession Number: BC065198
• Gene Symbol: LUC7L
• Gene ID (NCBI): 55692
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa.