LTF Monoclonal antibody, PBS Only

LTF Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D1G3

Cat no : 68509-1-PBS

Synonyms

Lactoferrin, HLF2, Growth-inhibiting protein 12, GIG12, GIG 12

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Validation Data Gallery

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  WB analysis of human saliva using 68509-1-Ig (same clone as 68509-1-PBS)

  human saliva were subjected to SDS PAGE followed by western blot with 68509-1-Ig (Lactoferrin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68509-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 68509-1-Ig (same clone as 68509-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68509-1-Ig (LTF antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68509-1-PBS in a different storage buffer formulation.

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Product Information

68509-1-PBS targets LTF in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: LTF fusion protein Ag33645
• Full Name: lactotransferrin
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 78 kDa
• GenBank Accession Number: BC015822
• Gene Symbol: LTF
• Gene ID (NCBI): 4057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).