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LTF Monoclonal antibody

LTF Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3D1G3

Cat no : 68509-1-Ig

Synonyms

Lactoferrin, HLF2, Growth-inhibiting protein 12, GIG12, GIG 12

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using LTF Monoclonal antibody (68509-1-Ig)

    WB analysis of human saliva using 68509-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 68509-1-Ig (Lactoferrin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using LTF Monoclonal antibody (68509-1-Ig)

    IHC staining of human skin cancer using 68509-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68509-1-Ig (LTF antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman saliva
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68509-1-Ig targets LTF in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen LTF fusion protein Ag33645
Full Name lactotransferrin
Calculated Molecular Weight 80 kDa
Observed Molecular Weight78 kDa
GenBank Accession NumberBC015822
Gene Symbol LTF
Gene ID (NCBI) 4057
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).

Protocols

Product Specific Protocols
WB protocol for LTF antibody 68509-1-IgDownload protocol
IHC protocol for LTF antibody 68509-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols