LRRFIP1 Recombinant antibody, PBS Only

• 

  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84778-4-RR (same clone as 84778-4-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using LRRFIP1 antibody (84778-4-RR, Clone: 242161C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

LRRFIP1 {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 84778-4-PBS

Clone No.242161C9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1

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Product Information

84778-4-PBS targets LRRFIP1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LRRFIP1 fusion protein Ag34760
• Full Name: leucine rich repeat (in FLII) interacting protein 1
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC108914
• Gene Symbol: LRRFIP1
• Gene ID (NCBI): 9208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q32MZ4
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).