LRRFIP1 Recombinant antibody, PBS Only (Detector)

LRRFIP1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242161F10

Cat no : 84778-2-PBS

Synonyms

GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1

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Validation Data Gallery

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  WB analysis using 84778-2-RR (same clone as 84778-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-2-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01600-1

  Sandwich ELISA standard curve of MP01600-1, Human LRRFIP1 Recombinant Matched Antibody Pair - PBS only. 84778-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34760. 84778-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL

• 

  Sample test of MP01600-1

  The mean LRRFIP1 concentration was determined to be 146.23 ng/mL in Raji cell extract based on a 1.4 mg/mL extract load, 70.95 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 131.01 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP01600-1

  Cytometric bead array standard curve of MP01600-1, LRRFIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84778-1-PBS. Detection antibody: 84778-2-PBS. Standard: Ag34760. Range: 0.313-40 ng/mL.

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  Cytometric bead array standard curve of MP01600-2

  Cytometric bead array standard curve of MP01600-2, LRRFIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84778-3-PBS. Detection antibody: 84778-2-PBS. Standard: Ag34760. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84778-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84778-2-RR against Human LRRFIP1 were performed. The affinity constant is below 1 pM.

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Product Information

84778-2-PBS targets LRRFIP1 as part of a matched antibody pair:

MP01600-1: 84778-1-PBS capture and 84778-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP01600-2: 84778-3-PBS capture and 84778-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LRRFIP1 fusion protein Ag34760
• Full Name: leucine rich repeat (in FLII) interacting protein 1
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: BC108914
• Gene Symbol: LRRFIP1
• Gene ID (NCBI): 9208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).