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LRRFIP1 Recombinant antibody

LRRFIP1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242161F10

Cat no : 84778-2-RR

Synonyms

GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LRRFIP1 Recombinant antibody (84778-2-RR)

    WB analysis using 84778-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84778-2-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84778-2-RR

    Affinity and Kinetic Characterization of 84778-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84778-2-RR against Human LRRFIP1 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inA549 cells, Raji cells, HEK-293 cells, COLO 320 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84778-2-RR targets LRRFIP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen LRRFIP1 fusion protein Ag34760
Full Name leucine rich repeat (in FLII) interacting protein 1
Observed Molecular Weight150-170 kDa
GenBank Accession NumberBC108914
Gene Symbol LRRFIP1
Gene ID (NCBI) 9208
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Protocols

Product Specific Protocols
WB protocol for LRRFIP1 antibody 84778-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols